Enhancing PCa cryotherapy using TRAIL sensitization.

[bert]

.
Enhancing PCa cryotherapy using tumor necrosis factor related apoptosis-inducing ligand (TRAIL) sensitization in an in vitro cryotherapy model.

- Abstract

Friday, September 4, 2009

---- Cryotherapy is a minimally invasive treatment for prostate cancer. Complete ablation of cancer tissue some times fails and results in disease recurrence. In this study we investigate the effect of TRAIL as a sensitizing agent to enhance the effects of cryotherapy on prostate cancer cells. Prostate cancer cells were cooled using Endocare cryo-system to mimic temperatures achieved during clinical cryotherapy. The effects of TRAIL, cryotherapy or combination of both treatment on DU-145 and PC-3 were evaluated. Viability and mode of cell death was assessed following treatment. Cryotherapy did not result in complete cell death at temperature -40 degrees C. Cells died by both necrosis and apoptosis. Cells which survived freeze-thaw cycle became more sensitive to a second freezing injury. TRAIL resulted in minimal cell death. Concomitant treatment of the tumour cells with TRAIL and cryotherapy resulted in complete loss of viability at -10 and -20 degrees C. Cell death was mainly due to marked increase in necrosis. Our finding demonstrates that combined treatment of TRAIL and cryotherapy represent a novel approach to increase the sensitivity to cryotherapy. This combined approach may be feasible for locally advanced prostate cancer.


Written by: Ismail M, Morgan R, Harrington K, Davies J, Pandha H.
Postgraduate Medical School, Guildford, UK.

Reference: Cryobiology. 2009 Oct;59(2):207-13.

PubMed Abstract --- PMID:19646431

-------
Bert ..... :bert: